Atmospheric Pressure Plasma Irradiation Facilitates Transdermal Permeability of Aniline Blue on Porcine Skin and the Cellular Permeability of Keratinocytes with the Production of Nitric Oxide

The transdermal delivery system of nutrients, cosmetics, and drugs is particularly attractive for painless, noninvasive sustainable release. Recently, atmospheric pressure plasma techniques have been great interest to improve the drug absorption rate in delivery. Currently, plasma-mediated changes lipid composition stratum corneum are considered a possible mechanism increase permeability. Nevertheless, its molecular cellular mechanisms largely confined still veiled. Herein, we present effects cold on transmission porcine skin permeability keratinocytes further demonstrate production nitric oxide from keratinocytes. Consequently, argon irradiation 60 s resulted 2.5-fold higher aniline blue dye compared nontreated control. In addition, plasma-treated showed an increased high-molecular-weight molecules (70 150 kDa) with oxide. Therefore, these findings suggest promoting effect low-temperature absorption, even molecules. Moreover, plasma-induced likely regulate epidermal layer.